Journal: Pharmacological reports : PR

Article Title: Anticancer effect of nor-wogonin (5, 7, 8-trihydroxyflavone) on human triple-negative breast cancer cells via downregulation of TAK1, NF-κB, and STAT3

doi: 10.1016/j.pharep.2019.01.001

Figure Lengend Snippet: Proliferation and viability of TNBC cells and non-tumorigenic breast cells after treatment with nor-wogonin and structurally related compounds. A. Chemical structures of nor-wogonin, wogonin, and wogonoside. B. Effects of nor-wogonin (5–80 μM), wogonin (100 μM), and wogonoside (100 μM) on the proliferation of TNBC cells (MDA-MB-231, BT-549, HCC70, and HCC1806) and non-tumorigenic breast cells (MCF-10A and AG11132) were determined by BrdU incorporation assays. Dimethyl sulfoxide (DMSO vehicle) was used as a negative control. C. The cytotoxic effects of nor-wogonin (5–80 μM), wogonin (100 μM), and wogonoside (100 μM) on TNBC cells (MDA-MB-231, BT-549, HCC70, and HCC1806) and non-tumorigenic breast cells (MCF-10A and AG11132) were determined by trypan blue exclusion assays. DMSO was used as a vehicle control. Data are expressed as the means ± SD based on three independent experiments.

Article Snippet: Human TNBC cell lines (MDA-MB-231, BT-549, HCC70, and HCC1806) and non-tumorigenic breast cell line (MCF-10A) were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: BrdU Incorporation Assay, Negative Control, Control

Journal: Cell metabolism

Article Title: Targeting Breast Cancer Stem Cell State Equilibrium through Modulation of Redox Signaling

doi: 10.1016/j.cmet.2018.06.006

Figure Lengend Snippet: (A–E) E- and M-BCSCs in SUM149 (A), HCC1806 (B), MCF7 (C) and T47D (D) BC cells treated with 2DG (10 and 20 mM, 40h) and effects of 2DG to induce apoptosis in M-BCSCs or bulk tumor cells (E). *, **: P<0.05 or 0.01 (vs. no 2DG).

Article Snippet: Cell Labeling and Flow Cytometry To determine the effect of drug treatment on M- and E-BCSCs in basal BC cell lines, SUM149 or HCC1806 following various treatment were digested by 0.25% Trypsin-EDTA, re-suspended in HF buffer (HBSS plus 2% FBS) at 2×10 5 cells/100 μl and incubated with antibodies against human CD24 (PE-Cy7-conjugated, 1:100 from BD for SUM149 or 1:75 from Biolegend for HCC1806) and CD44 (APC-conjugated, 1:200, from BD) in cold room for 30 minutes.

Techniques:

Journal: Cell metabolism

Article Title: Targeting Breast Cancer Stem Cell State Equilibrium through Modulation of Redox Signaling

doi: 10.1016/j.cmet.2018.06.006

Figure Lengend Snippet: (A, B) SUM149 BC cells treated with 2DG/H2O2 for 1.5h (A) or NAC for 20h (B) was analyzed by p-ACC (S79) and HIF1α antibodies.

Article Snippet: Cell Labeling and Flow Cytometry To determine the effect of drug treatment on M- and E-BCSCs in basal BC cell lines, SUM149 or HCC1806 following various treatment were digested by 0.25% Trypsin-EDTA, re-suspended in HF buffer (HBSS plus 2% FBS) at 2×10 5 cells/100 μl and incubated with antibodies against human CD24 (PE-Cy7-conjugated, 1:100 from BD for SUM149 or 1:75 from Biolegend for HCC1806) and CD44 (APC-conjugated, 1:200, from BD) in cold room for 30 minutes.

Techniques:

Journal: Cell metabolism

Article Title: Targeting Breast Cancer Stem Cell State Equilibrium through Modulation of Redox Signaling

doi: 10.1016/j.cmet.2018.06.006

Figure Lengend Snippet: (A–C) Contents of E- (A) and M- (B) BCSCs in SUM149 treated with AUR, BSO or AUR+BSO with or without NAC for 24h and the effects of Trig to sensitize E-BCSCs to AUR (C). *, **: P<0.05 or 0.01 vs. untreated. NS: not significant.

Article Snippet: Cell Labeling and Flow Cytometry To determine the effect of drug treatment on M- and E-BCSCs in basal BC cell lines, SUM149 or HCC1806 following various treatment were digested by 0.25% Trypsin-EDTA, re-suspended in HF buffer (HBSS plus 2% FBS) at 2×10 5 cells/100 μl and incubated with antibodies against human CD24 (PE-Cy7-conjugated, 1:100 from BD for SUM149 or 1:75 from Biolegend for HCC1806) and CD44 (APC-conjugated, 1:200, from BD) in cold room for 30 minutes.

Techniques:

Journal: Cell metabolism

Article Title: Targeting Breast Cancer Stem Cell State Equilibrium through Modulation of Redox Signaling

doi: 10.1016/j.cmet.2018.06.006

Figure Lengend Snippet: (A, B) SUM149 treated with AUR (0.5 μM) or BSO (1mM) for 24h were labeled with Annexin V and examined for the content of E-BCSCs (A) and Annexin V+ cell ratio in ALDH+ and ALDH− cell populations (B).

Article Snippet: Cell Labeling and Flow Cytometry To determine the effect of drug treatment on M- and E-BCSCs in basal BC cell lines, SUM149 or HCC1806 following various treatment were digested by 0.25% Trypsin-EDTA, re-suspended in HF buffer (HBSS plus 2% FBS) at 2×10 5 cells/100 μl and incubated with antibodies against human CD24 (PE-Cy7-conjugated, 1:100 from BD for SUM149 or 1:75 from Biolegend for HCC1806) and CD44 (APC-conjugated, 1:200, from BD) in cold room for 30 minutes.

Techniques: Labeling

Journal: Cell metabolism

Article Title: Targeting Breast Cancer Stem Cell State Equilibrium through Modulation of Redox Signaling

doi: 10.1016/j.cmet.2018.06.006

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Cell Labeling and Flow Cytometry To determine the effect of drug treatment on M- and E-BCSCs in basal BC cell lines, SUM149 or HCC1806 following various treatment were digested by 0.25% Trypsin-EDTA, re-suspended in HF buffer (HBSS plus 2% FBS) at 2×10 5 cells/100 μl and incubated with antibodies against human CD24 (PE-Cy7-conjugated, 1:100 from BD for SUM149 or 1:75 from Biolegend for HCC1806) and CD44 (APC-conjugated, 1:200, from BD) in cold room for 30 minutes.

Techniques: Recombinant, Inhibition, Luciferase, Reporter Gene Assay, RNA Sequencing Assay, shRNA, Software

Journal: Nucleic Acids Research

Article Title: In situ tracking of glycoRNAs on single-cell surface to reveal RNA heterogeneity and transport mechanism

doi: 10.1093/nar/gkag362

Figure Lengend Snippet: GLINT visualization of glycoRNA content changes during breast cancer malignant transformation. ( A–E ) Confocal images and single-cell fluorescence histograms of MCF-10A, MCF-7, BT474, SK-BR-3, MDA-MB-361, BT549 HS578t, MDA-MB-436, HCC1806, and MDA-MB-231 cells obtained via GLINT combined with T RNA-U1 , T RNA-U3 , T RNA-U35a , T RNA-Y5 , T RNA-U8 .

Article Snippet: MCF-7cells were obtained from China Center for Type Culture Collection (CTCC), while MCF-10A, SK-BR-3, MDA-MB-231, BT549, Hs578T, HCC1806, MDA-MB-361, and MDA-MB-436 cells were purchased from Procell.

Techniques: Transformation Assay, Single Cell, Fluorescence